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KMID : 0361120080220010041
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Korean Journal of Transplantation
2008 Volume.22 No. 1 p.41 ~ p.48
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The Effect of Intra-portal Infusion of Glucose- insulin-potassium (GIK) Solution on the Energy Metabolism during Cold Preservation in a Small- animal Model for Liver Transplantation
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Ha Tae-Yong
Hwang Shin
Lee Sung-Gyu
Hong Hea-Nam
Ahn Chul-Soo
Kim Ki-Hun
Moon Deok-Bog
Song Gi-Won
Jung Dong-Hwan
Ryu Je-Ho
Choi Nam-Kyu
Kim Kwan-Woo
Ko Kyoung-Hun
Li Guang
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Abstract
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Purpose: The effect of intra-portal infusion of glucose-insulin-potassium (GIK) solution on the energy metabolism during cold preservation was investigated using a small- animal liver transplantation model.
Methods: Fifteen white rats were divided into 3 groups: the group A (feeding group) were fed normally before experiment. The group B (fasting group) and group C (GIK group) were fasted from 3 days before experiment, by which acute nutritional deficiency state was induced. In group A and B, the whole liver was procured after intra-portal perfusion of HTK solution and serial liver biopsies were performed during the cold preservation period with 4oC HTK solution. In group C, intra-portal GIK solution infusion for 1 hour preceded liver graft harvest. From the liver tissues, the relative intracellular glycogen contents and the ATP concentration were measured.
Results: Relative glycogen contents in group A were 100% at 0 h, 64.6% at 2 h, 54.9% at 4 h, and 16.2% at 8 h; 10.3%, 8.3%, 4.9% and 0%, respectively in group B; 109.2%, 96.9%, 54.2% and 9.7%, respectively in group C. There was a temporary supercharge of ATP level in group C only at 0 h. Apoptosis was less expressed in group C comparing with group A and B.
Conclusion: Rapid intra- portal infusion of GIK solution could make intrahepatic glycogen content fully restored to the normal level. Considering that intracellular glycogen is the main energy source during immediate post-transplant period, its restoration may contribute to improvement of post-transplant graft function.
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KEYWORD
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Liver transplantation, Glycogen, Energy metabolism, Cold preservation
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